BULLETIIV OF THE CHARLESTON MUSEUM »»' Edited By PAUL M. REA Vols. I-V LIBRARY IQO'^ 1Q09 NEW YORK lyoo— lyuy botanical Qardb CHARLESTON, S. C 1909 printed for the museum by The Daggett Printing Co. charleston, s. c. Under the Auspices of the College of Charleston Director Paul M. Rea Librarian Laura M. Bragg Honorary Curators Wm. G. Mazyck Conchdogy Daniel S. Martin Geology Arthur T. Wayne Ornithology Nathaniel W. Stephenson Art The Charleston Museum was in existence as early as 1777 under the auspices of the Charleston Library Society, was transferred to the Literary and Philosophical Society of South Carolina in 1815, to the Medical School in 1828, and to the College of Charleston in 1850. In 1907 a building was provided by the City and the name changed to The Charleston Museum. The Museum is dependent upon dues from members and private sub- scriptions for all permanent improvements, for increase of the collections, and for maintenance of its educational and scientific work. The membership fees are:— Annual Members $ 10 Patrons $ 500 Sustaining Members 25 Benefactors 1000 The privileges of members include admission on pay days, tickets to members' lecture courses, and copies of Museum publications. The Bulletin of the Charleston Museum is published monthly, from October to May, by the Museum and is entered at the Post Office at Charleston, S. C, as second class matter. The Bulletin of the College of Charleston Museum is publishetl monthly from Oct- ober to Alay and is devoted to descriptions of exhibits, reports of lectures, and notes from the Museum. Application made at the post office at Charleston, S. C. for entry as second-ciass matter. BULLETIN OF THE COLLEGE OF CHARLESTON MUSEUM Editeo by PAUL ^L. RE A CURATOR OF THE MUSEUM AND PROFESOR OF BIOLOGY Vol. 1 Charleston, S. C, April, 1905 No. l MUSEUMS OF NATURAL HISTORY AND THEIR WORK = Until very recently the botany taught in our schools and colleges was devoted almost exclusively to identification and systematic arrangement of the flowering plants and ferns. Nov*' all this is changed and a ''new" botany is firmly estab- lished which studies the principles underlying the structure and behavior of plants and relegates details of classification to specialists. Zoology underwent a similar but perhaps more gradual transformation some time ago and in both of these cases the cause is to be sought in a general change in the aims of biology. Each phase of this movement is conspiciously shown in the history of our natural history museums. In the latter part of the eighteenth century the study of natural history received a tremendous impetus from the la- bors of the great Swedish naturalist, Linnaeus. At that time there was great confusion in the nomenclature and classifi- § cation of both animals and plants. Linnaeus showed untiring 1 "5 zeal in collecting and great genius in classifying. To each J * A public lecture by Profesor Rea on the occasion of the opening of Manigault Hall as a lecture room. form he gave two names, a generic and a specific. Thus the common cat is called Fells doinestica and the lion F. leo, while the dog is Cants familiaris. To these binom- ial designations it is customary to add the abbreviated name of the person who first used them. So the full scientific name of the cat is Felis domestica Linn. The systematic work of Linnaeus was continued by his followers and great collections were built up which had an increasing value as time went on, for sometimes doubt would arise as to the real identity of a form or whether forms de- scribed under different names were not after all the same. In such cases the necessity of comparing the original speci- mens, or types, is obvious. In order to care properly for natural history collections and render them easily accessible museums were formed and of course the arrangement of the museums was "systematic," i. e. according to the scheme or system of classification adopted. The chief aim of biology at this time was to name and file away the largest possible number of forms. Structure vs^as carefully studied so far as it contributed to this end but, unfortunately, the investigation was considered complete as soon as the specimen was mounted and labeled. Museums were indispensable tools of science in this period and served as books of reference do in our libraries. The publication in 1859 of Darwin's Origin of Species checked the scramble after new species and stimulated a search for more fundamental principles in biology. Field and museum alike were searched for evidence of variation and complaints began to arise because museum collections were inadequate for such studies. Hitherto museums had ')een quite content with a few duplicates of convenient specimens and the forms which varied from the type had been an un- mitigated nuisance to naturalists because of the difficulty of classifying them. Now, not only are many duplicates de- sired for study of variations but adaptations of every descrip- tion, homologies, and life-histories are eagerly sought and v/ith all these come opportunities such as the older mus- eums never knew. Let us, then, look somewhat minutely into the work of a modern museum of natural history that we may better appreciate its achievements and condone its shortcomings. As soon as a specimen is received at the museum it is re- ferred to tlie proper authority for identification. Members of the staff of large museums are usually able to identify speci- mens but they sometimes have to be sent away to specialists. As soon as the specimen is identified it receives an accession number which is marked upon it in the most permanent man- ner possible. Under this number in an accession book is en- tered all the available information about the specimen, which is then placed in the study collection or on public exhibition. From the accession record a catalogue card is prepared and filed alphabetically for ready reference. Beside the accession number and the name of the specimen the card bears a loca- tion mark which shov/s where the specimen is at any time. The larger museums have both study or research collec- tions and exhibition collections but many of the smaller insti- tutions are confined to the latter. The study collection is, however, the basis of all active work in a museum. It does not make an impressive appearance because most of the speci- mens are unmounted and are packed away in trays and draw- ers. Yet here are many of the invaluable types, ail the dupli- cates, and all specimens not on exhibition. It is a great mis- take for a museum to exhibit all its material at one time, for very much more interesting and instructive exhibits can be prepared by judicious selection of specimens. The study col- lection is used for research and studj^ by all properly quali- fied persons and it should be conveniently arranged and pro- vided with well lighted tables and a good working library. The organization of the exhibition collections is a more complex problem and demands sound judgment as well as the highest degree of technical skill. In order to accomplish the best results a specimen should never be exhibited except for a definite purpose and then no pains should be spared to make the fulfillment of that purpose as complete as possible. Many things contribute to this result. One which requires special care is designing cases which give ade- quate protection to specimens and at the same time de- tract as little as possible from their appearance. Wherever it can be done each side of a case should consist of one sheet of glass only and the corners should show only as much wood as is necessary. Since the lighting of exhibition rooms is pref- erably from skylights or windows high in the wall, the tops of cases should be made of glass. The doors must be dust and insect proof at all costs. In order to facilitate a rear- rangement of material at any time "unit" cases are often used which are interchangeable throughout the museum. When skins are to be stuffed the very best taxidermy is required to avoid mere caricatures which convey more false than true impressions of the unfortunate animal. The best mounting includes the reproduction of the typical en- vironment of the animal. So bison are represented on a bit of prairie and beavers in their forest haunts. In this work there are many pitfalls for the novice and perhaps nowhere is the difference between excellence and mediocrity more conspicuous. When specimens are preserved in fluid many shapes and sizes of receptacles are needed. Cylindrical jars were formerly the only shape available but they distort the appearance of specimens by acting as lenses. Recently rectangular jars have been produced which obviate this dif- ficulty. Snakes and worms coiled in bottles show only one side and are often best displayed in tubes. Brilliantly colored forms like squids, fishes, and lizards inevitably fade whether they are stuffed or preserved in fluids. The most satisfactory method of exhibiting these forms is by the use of casts and models colored accurately from fresh specimens. The use of models also gives an opportunity of getting more natural po- ses and environment. In this way lizards can be shown on typical desert rocks. Wherever this method is used the act- ual specimens are preserved for reference in the study col- lection. When the specimens are finally prepared and arranged the making of labels is in order. Before even beginning to write labels one should know enough to write a book about thes pec- imens. Then the facts most essential for the purpose in hand must be selected and arranged in a form pleasing to the eye, for poor typography may spoil the whole effect. Labels are always in danger of becoming separated from their speci- mens, but since both bear the accession number they are easi- ly brought together again. It is often desirable to put the accession number on the shelf where each specimen belongs to facilitate restoring the specimens after temporary removal. The accession number is thus the key to the whole record of each specimen and if it becomes obliterated the specimen is sometimes almost worthless. It may well be asked, What is the purpose of museum ex- hibits that such extraordinary care is required in the details of their preparation? It is always to attract and hold at- tention until a definite and lasting impression can be made on the mind of the observer. Museums are continually striv- ing to correct the tendency which most people have to go through the whole collection and get a glimpse of each speci- men all in a single visit. Nothing is more fatiguing than ''doing" museums in this manner and the confused impres- sion which results from the ordeal is a poor return for the time and energy consumed in forming it. If you would real- ly enjoy a visit to a museum go and see a very few things, learn enough to feel personally acquainted with them, and come away before you are tired. Modern museums encour- age this practice in two ways. They prepare special exhibits which are made the chief attractions for limited periods of time and they frequently arrange lectures based on these special exhibits. Such lectures are especially valuable in providing a definite object for each visit. The preparation of special exhibits involves much labor and expense, and the museum naturally wishes to know the relative attractive value of various kinds of exhibits. For this purpose regis- tering turnstiles are used to record the number of visitors. A brief account of the College of Charleston Museum may be appropriately given here for the purpose of showing the work which it is fitted to do and the difficulties w^hich confront it. Such an account vv^ill complete the general dis- cussion of our subject by disclosing the vicissitudes of many of the natural history museums of this country. The Muse- um was organized in 1850 as a result of the interest in natu- ral history aroused by Louis Agassiz and that famous coterie of scientific men who lent luster to the culture of Charleston in the first half of the nineteenth century. The Museum re- ceived the older private collections of Elliot, Holbrook, Aud- ubon, Tuomey, and Holmes. At about this time museums of natural history were established in a great many other col- leges in this country. The animus of them all was the old systematic natural history and they had no sooner gotten fairly started than the interest in that form of science began to wane under pressure of the new Darwinian biology. Most of these museums were unable to adapt themselves to the new conditions and so have gradually drifted into neglect and de- cay. This Museum was fortunately preserved from the com- mon fate by the devotion of its curators, Professor Holmes and Dr. Manigault, under v/hose care the collections have greatly increased and come down to us fairly v/ell preserved, in spite of war, fire, and earthquake. To-day this is considered the largest museum in the South. The study collection in- cludes the Stephen Elliott herbarium, rich in types and fre- quently consulted by botanists from all parts of the country. This herbarium is now being renovated and a printed report on its present condition will soon be published. A suitable safe is needed to protect this herbarium from the ravages of insects and danger of fire. The exhibition specimens are nearly all of very good quality and this is especially true of the mammals. To the work of Professor Ashley, recently curator of the Museum is due the beginning of a modern ar- rangment of the collections but this work is very difficult because of the crowded ill-lighted rooms, the difficulty of moving many of the cases, and the entire lack of storage room. Hitherto no proper accession record has been kept but a beginning of this work is now being made. A card cata- logue was prepared by Professor Ashley and is being contin- ued. Many of the labels in this Museum are more than thirty years old and need to be renewed. Museums obtain new material by gift, by purchase, and by exchange. A museum should always possess an abun- dance of local material for exhibition. If a quantity is also a- vailable for exchange it is quite as valuable as an increased ap- propriation. Charleston is situated in the midst of phosphate deposits which yield a rich variety of fossils and the Museum has one very handsome case of these but it could easily have the largest and most complete collection of the sort in this coun- try. There are many barrels of these fossils in Charleston which have very little value to their owners. If these could be given to the Museum the best specimens would form a note- worthy exhibit and the remainder would bring to the Muse- um by exchange material which cannot be obtained by pur- chase. In addition to the routine work of accessioning the col- lections the present plans cf the Museum include the prepar- ation of special exhibits, provided with descriptive labels and supplemented by illustrated lectures in Manigault Hall. To encourage and assist regular visitors the Museum has un- der taken the publication of this monthly Bulletin, devoted to descriptions of exhibits, reports of lectures, and general information about the Museum. The Bulletin^ is intended to take the place of a museum guide for all visitors and to make public the work of the Museum. NOTES FROM THE MUSEUM The Museum is open to the public on Saturdays from 10 to 5 and on other week-days from 10 to 12 and 1 to 5. Chil- dren unaccompanied by an adult are admitted only on Satur- days. Professor Rea's lecture on "Museums of Natural History and Their Work" was illustrated with lantern slides and spec- imens of good and bad mounting from the Museum collections. One of the best examples of skillful taxidermy in the Muse- um is a group of English pheasants which was purchased this month and exhibited for the first time at the lecture. It in- cludes a cock, standing on an old stump, while the hen crouches almost out of sight in the tall grass of the bog and eight little chicks are busily looking for food. Each bird is in a perfectly natural pose and the group shows the habits as well as the form and coloring of the English pheasant. Visitors to the Museum should not fail to see this new exhibit. The need of a suitable lecture room in the Museum has been met by the recent alterations in Manigault Hall. A re- arrangement of the archaeological collections has secured a large floor space and the room has been provided with a plat- form with a reading desk and a long table for display of specimens, a sliding blackboard, and screens and electric lights for the use of a new projection apparatus which will be a prominent feature of the Museum lectures. This appar- atus uses an electric lantern for projecting on a screen both ^tereopticon and microscopic slides and makes it possible to show the living animals and plants in a drop of water. The Museum wishes to receive gifts of phosphate fossils in any quantity and of large garden slugs alive. 8 Sassard rtROs. I*Ri>rr. The Bulletin is publislied monthly from October to Alay by the College of Charles- ton and is devoted to descriptions of exhibits, reports of lectures, and notes from the Museum. Application made at the post office at Charleston, S. C. for entry as second- class matter. BULLETIN OF THE COLLEGE OF CHARLESTON MUSEUM Edited by PAUL M. REA CURATOR OF THE MUSEUM AND PROFESSOR OF BIOLOGY Vol. 1 Charleston, S. C, May, 1905 No. 2 CONTENTS Charleston Natural History Society - Page 10 Constitution - - - Page 11 S Theory of killing and preserving - - Page 15 Special directions for summer collecting - Page 17 CD O Charleston Natural History Society At the suggestion of Professor Rea, the Curator of the Museum, a meeting was held in the College Library May 11th. to consider plans for the formation of a natural history society. Fifteen students were present and after a general discussion of the purpose of the meeting it was unanimously decided to form a society and a committee was appointed to draft a constitution. In these days when so many of the old scientific societies have gone out of existence for lack of interest and support some apologj'- may be required for the formation of a new natural history organisation. To this end Professor Rea ex- plains that there are several students in college and other persons about Charleston who are interested in natural his- tory but who have no training in collecting and no way of caring for material v/hich they may get. It should be a part of the work of the Museum to stimulate and assist such per- sons and as a first step in this direction the Charleston Nat- ural History Society is formed under the auspices of the Museum as a means of bringing together persons who are interested in this subject and extending to them the facili- ties of the Museum and such instruction as it can give in methods of observing, collecting, identifying, and preserving specimens. One of the most valuable features of the society will be the exchange of experience among members and the accumulation of records of observations. The Museum will loan glassware and supply preserving and killing fluids and in return it is expected that most of the specimens ob- tained will be given to the Museum. The organisation of the societv is intended to be as plas- tic as possible in order that it may not interfere with other duties of members. The curator of the Museum is, e.r-offido, director of the Scientific work of the society and plans the work in consultation with an executive committee composed of the officers of the Society, to whom is given the carrying 10 out of the plans. The work of members consists in making and recording observations of natural history, and in collecting, killing, preserving, and identifying specimens. All specimens are deposited with the proper officers of the club and must in all cases be accompanied by a carefully prepared written report, which is not accepted by the society until approved by the director. Members are not required to accomplish any specified amount of work for the society but they must have at least one regular report accepted each six months. The meetings of the society are devoted to reports of mem- bers and scientific discussions. The membership is not con- fined to students of the college and ail persons interested in natural history arfe invited to join. CONSTITUTION Article I. Nams and Purpose Sec. 1. Name. The name of this organization shall be The Charleston Natural History Society. Sec. 2. Fnrpose. The purpose of the Society shall be the study of natural history and the development of the Col- lege of Charleston Museum. Sec. 3. Official Organ. The official organ of the Society shall be the Bulletin of The College of Charleston Museum. Article II. Officers Sec. 1. Names of Officers. The officers of the Society shall be: a Director, a President, a Vice-President, a Secretary, and a Treasurer. Sec. 2, Duty of the Director. It shall_ be the duty of the Director to plan the scientific work of the Society in con- sultation with the Executive Committee, of which he shall be the chairm.an, and to examine all reports of members. Sec. 3. Dutij of the Frcsidcnt. It shall be the duty of the President to represent the Executive Committee in car- 11 rying on the Scientific work of the Society, to preside at all meetings, and to perform all duties usually pertaining to this office. Sec. 4. Duty of the Vice-President. It shall be the duty of the Vice-President to perform all the duties of the Presi- dent in his absence. Sec. 5. Duty of the Secretary. It shall be the duty of the Secretary to keep minutes of all meetings of the Society and of the Executive Committee, to receive all reports of members, to submit the same to the Director for examina- tion, and to perform all duties usually pertaining to this office. Sec. 6. Duty of the Treasurer. It shall be the duty of the Treasurer to collect all moneys due the Society, to make all disbursements authorized by the Society, and to keep an account of all receipts and expenditures. It shall also be the duty of the Treasurer to receive and care for all properly prepared specimens deposited by members, and to place the same in the Museum when called upon by the Direct- or. It shall be the further duty of the Treasurer to keep a record of all supplies furnished to each member and of all specimens received from him. Sec. 7. Executive Committee. The officers of the Society shall constitute an Executive Committee, with the Director as chairman, and it shall be the duty of the committee to as- sist the Director in planning the scientific v/ork of the Society. It shall also be the duty of the Executive Committee to nom- inate, at least one week previous to each election, such active members as are best qualified for office. Article III. Election of Officers Sec. 1. Time of Election. Officers for the ensuing six months shall be elected at the regular meeting in May and in November. 12 Sec. 2. Mctliod of Election. The Curator of the Col- lege of Charleston Museum shall be, ex-officio, the Director of the Society. All other ofRcers shall be elected from the nominations of the Executive Committee by a majority of the votes cast. All elections shall be by ballot. Article IV. Members Sec. 1. Kinds of Members. Members shall be of four kinds: viz., Probationary, Active, Delinquent, and Honorary. Sec 2. Prohationanj Members. All new members shall be probationary and shall be required to file with the Secre- tary an approved report within six months. Upon doing this they shall become active members or by failing to do this they shall become delinquent members as hereinafter pro- vided. Any person may become a probationary member upon application to the Secretary and payment of the initiation fee to the Treasurer. Sec. 3. Active Mem.hers. All members who have filed with the Secretary at least one approved report within six months shall be active members and shall remain active mem- bers as long as they file with the Secretary at least one ap- proved report every six months. Sec. 4. Delinquent Members. All members who have not filed with ^e Secretary at least one approved report within six months shall be delinquent members and shall have no vote. Delinquent members become active upon filing with the Secretary an approved report. Delinquent mem- bers of six months* standing shall be dropped from the rolls, but may join as new members at any time. Sec. 5. Honorary Members. Any person may be nomi- nated by the Executive Committee for honorary membership and may be elected by two-thirds of the voting members present at any regular meeting. Honorary members shall be exempt from all fees and assessments. 13 Sec. 6. Reporfs of Memhers. All scientific work done by members for the the Society shall be presented in the form of a written report which shall be filed by the Secretary when it has been approved by the Director. Article V. Meetings. Sec. 1. Regular Meetings. Regular Meetings shall be held once each month during the college year at a time to be decided by a majority of the voting members. Sec. 2. Special Meetings. Special Meetings may be called at any time at the discretion of the President or the Director, or at the request of any tv/o members. Sec. 3. Quorums. The voting members of the Society shall consist of the active and probationary members. A quorum for election of officers or for any financial question shall consist of half of the voting members. A quorum of the Executive Committee shall consist of the Director and two others. Article VI. Fees and Assessments. Sec. 1. Initiation Fee. All applicants for membership shall pay to the Treasurer an initiation fee of fifty cents, which shall be given to the Director for the use of the Mu- seum. Sec. 2. Assessments. Members may be assessed for any purpose by two-thirds of the voting members present at any regular meeting. Sec. 3. Fines. Any member failing to pay any legal assessment within one month shall be fined the amount of of the assessment, and failing to pay both the assessment and the fine within six months shall be dropped from the rolls, and shall be ineligible to join as a new member until all arrears shall have been paid. 14 Article VII. Amendments. Sec. 1. Constitutional Amendments. This constitution may be amended upon a month's notice by two-thirds of the voting members present. Sec. 2. Amendments to Bij-Laws. The By-Laws may be amended by two-thirds of the voting members present at any regular meeting. THEORY OF KILLING AND PRESERVING. Every collector of biological specimens should thor- oughly understand the general theory of killing and pre- serving. On this depends net only the intelligent and successful use of the special methods that have been devised for particular cases, but what is far more important, the ability to devise methods adapted to any form. Kitlli/f/. No method of killing is satisfactory which dis- torts or mutilates the specimen, at least in parts which are intended for study. For this reason animals which are to be dissected or stuffed should not be shot if they can be captur- ed in any other way. Chloroforming is the most convenient method of killing reptiles, birds, and mammals, though drowning is an admirable method when there is no objection to wetting the feathers or hair. The simplest way of killing small animals is by dropping them into strong (70-95'/' ) alco- hol, but many forms contract so strongly under this treat- ment as to make it entirely unsatisfactory. In these cases tv/o courses of procedure are open. Death may be inflicted so suddenly that the animal actually has not time to con- tract or anaesthesia may be used to induce complete relaxa- tion and unconsciousness before the animal is killed. The former method, sudden killing, may be accomplished by pouring strong acid mixtures or corrosive sublimate, hot, if necessary, over the specimens while they are relaxed. Hy- 15 droids may be killed in this way fully expanded. When the second method is followed anaesthesia may be induced in various ways. Earthworms soon become unconscious and perfectly relaxed in dilute (30-50'/' ) alcohol, and may be killed in strong alcohol. The most generally useful anaes- thetic is the vapor of chloroform. Specimens should never come in contact with the liquid. Marine animals are very easily and successfully killed by immersion in fresh water and vice versa. Fixing. As soon as an animal dies decomposition pro- cesses begin to alter the structure of the tissues and in order to prevent these changes the natural structure must be "fixed." Nearly all sudden killing fluids fix tissues at the same time. After the use of anaesthetics, however, tissues should be fixed as soon as possible by immersion in the pres- erving medium. Successful fixation depends on bringing all the tissues quickly in contact with the fixing agent. To facili- tate this an incision may be made to admit the fluid into large specimens. Fixation is synonymous with hardening and dur- ing the process specimens must be held in the position in which they are wanted permanently. Preserving. In biological practice two preserving media are in general use. Tissues may be kept indefinitely in 70% alcohol or, if they are thoroughly fixed, even in the vapor of al- cohol. Fresh tissues, however, contain much water and should be immersed in several times their bulk of full strength alcohol, which must be renewed after a few days if the specimens are large. Formalin is sold in 40/' solution, 5-7V' of which in water is sufficient to preserve animals as large as a cat. For smaller forms 1-4% formalin may be used. Formalin is much more economical than alcohol, does not extract so much water from the specimens, and does not evaporate so quickly and should therefore be used for preservation of all large animals. For smaller forms alcohol is probably simplest to use. Summary. Small animals should be killed, fixed, and 16 preserved by immersion in strong alcohol but if this causes contraction and distortion they should first be anaesthetized and then fixed as soon as they are unconscious and relaxed. Large animals should be anaesthetized and then im.mersed in 4-7% formalin for fixing and preserving. All soft or dis- torted specimens should be immediately thrown a\vay. When peculiar difficulties present themselves study these general principles carefully and experiment with all the means at hand until a successful method is attained; then make careful notes of your experiment. Special Directions for Summer Collecting The Museum wishes to keep on hand for dissection sev- eral dozen of each of the following forms and each member of the Natural History Society should m.ake these the basis of his work during the summer. Eartliworms. Where earthworms are abundant speci- mens the size of a lead pencil mxay bel ooked for on the sur- face of the ground at night. Use a light and avoid jarring the ground. Anaesthetize in 30-50 /t alcohol and transfer at once to 95*// alcohol, holding each worm extended a moment till hardened. Select the perfectly straight specimens, roll them up in a strip of cloth and preserve in 95V( alcohol, re- renewed after three days. Make full record of localities, habits, behavior, and methods of capturing and putting up. Garden Slugs. Anaesthetize in SOV^ alcohol. A capsule of coagulated slime will form about each specimen. Cut this and roll the animal out and preserve in 95'/< alcohol. Experiment to find a method of killing these animals fully relaxed. Make careful report on the living animals and on methods of putting up. Crmufishes. Kill, fix, and preserve in strong alcohol, changed once after three days, or in 39' formalin. Full written report. GrassJwp2)ers and CorlroacJies. Take only the largest specimens and kill, fix, and preserve in strong alcohol or 3% 17 formalin. Myriapods or Tliousandlegs. Drop all kinds into 70% alcohol. Frogs. Chloroform, fix in natural position and preserve in strong- alcohol, renewed once after three days, or in 4% formalin. Lizards. Treat like frogs, using special care to fix straight Salamanders, Neiuts, Mud Puiwies, and all other Tailed AmpJiihians. Those that breath by lungs may be chloro- formed. Those that breath by gills may be immersed in Avater containing salt, or alcohol or formalin may be added slowly to the water. Fix carefully with all parts straight. Fill the abdominal cavity with fixing fluid. Preserve in 6Vf formalin or in strong alcohol, renewed after three days and again after ten days. In spite of all popular reports none of these ampliihia are imisonons. HaU-groivn Kittens. Take only clean specimens of the right size. Kill by drowning or chloroform, fix carefully and preserve in 7% formalin. All specimens should be stored in vvide mouthed bottles, tightly stoppered, glass fruit jars, earthenv/are jars, or tin pails tightly covered. All except the smaller specimens may be wrapped in white cotton cloth to advantage. Notes may be written on this cloth in lead pencil. The Museum will pay transportation charges on good material. This must be au- thorized in advance, however, after full written description. SASSARD BROS. PRINT 18